1TTO

Crystal structure of the Rnase T1 variant R2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Collection date	2004-04-20
Spacegroup name	P 61
Unit cell lengths	56.524, 56.524, 159.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.950 - 2.100
Rwork	0.187
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q9e
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	278	PEG4000, tris-Cl, calcium chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K