1TT7
Crystal structure of Bacillus subtilis protein yhfP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.219, 133.546, 166.335 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.19111 |
| Rwork | 0.187 |
| R-free | 0.26192 |
| Structure solution method | SAD |
| RMSD bond length | 0.035 |
| RMSD bond angle | 3.006 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.128 | 0.128 |
| Number of reflections | 408512 | |
| Completeness [%] | 99.1 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 5% PEG4K, 0.1M BisTris, 0.16M MgCl2, 5% Glucose, 0.2M NAD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
