1TT0
Crystal Structure of Pyranose 2-Oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2552 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.920, 101.690, 135.630 |
| Unit cell angles | 90.00, 90.95, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.13407 |
| Rwork | 0.134 |
| R-free | 0.17122 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.775 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.113 | 0.344 |
| Number of reflections | 232099 | |
| <I/σ(I)> | 8 | 2.8 |
| Completeness [%] | 92.6 | 62.8 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 298 | mme PEG 2000, sodium acetate, magnesium chloride, Ta6Br12, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
