1TSW
THYMIDYLATE SYNTHASE R179A MUTANT
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | AREA DETECTOR |
Collection date | 1991-01-24 |
Detector | SIEMENS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 78.300, 78.300, 243.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 2.500 |
R-factor | 0.18 |
Rwork | 0.180 |
RMSD bond length | 0.005 |
RMSD bond angle | 24.300 * |
Data reduction software | X-GEN |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 2.550 |
Rmerge | 0.140 |
Total number of observations | 97328 * |
Number of reflections | 12977 |
Completeness [%] | 84.0 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 | pH 7.4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2-7 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 40 (mM) | |
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | ammonium sulfate | 0.5-2.0 (%sat) | |
5 | 1 | reservoir | phosphate | ||
6 | 1 | reservoir | dithiothreitol | ||
7 | 1 | reservoir | EDTA |