1TRP

X-RAY CRYSTALLOGRAPHIC AND CALORIMERIC STUDIES OF THE EFFECTS OF THE MUTATION TRP 59 TYR IN RIBONUCLEASE T1

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	49.150, 48.150, 40.150
Unit cell angles	90.00, 90.35, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.16
RMSD bond length	0.005
RMSD bond angle	1.240
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Total number of observations	10071 *
Number of reflections	6347 *
Completeness [%]	84.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	drop	2'GMP	10 (mM)
3	1	drop	sodium acetate	20 (mM)
4	1	drop	calcium acetate	2 (mM)
5	1	reservoir	MPD	51 (%(v/v))
6	1	reservoir	sodium acetate	20 (mM)