1TRI
THE CRYSTAL STRUCTURE OF AN ENGINEERED MONOMERIC TRIOSEPHOSPHATE ISOMERASE, MONOTIM: THE CORRECT MODELLING OF AN EIGHT-RESIDUE LOOP
Experimental procedure
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.780, 42.800, 68.840 |
| Unit cell angles | 90.00, 108.22, 90.00 |
Refinement procedure
| Resolution | 32.600 - 2.400 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.610 |
| Phasing software | X-PLOR |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.400 * | 2.400 * |
| Total number of observations | 15495 * | |
| Number of reflections | 7368 * | |
| Completeness [%] | 29 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 100 (mM) | |
| 2 | 1 | reservoir | 180 (mM) | ||
| 3 | 1 | reservoir | PEG6000 | 26 (%) | |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | 1 (mM) |
