1TR9
Structure of beta-hexosaminidase from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-21 |
Detector | MARRESEARCH |
Wavelength(s) | .9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.980, 79.378, 86.685 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.17182 |
Rwork | 0.170 |
R-free | 0.20314 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.239 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.150 | 0.283 |
Number of reflections | 31858 | |
<I/σ(I)> | 11.07 | 8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 20% PEG 5000, 0.1M BisTris pH 6.5, 0.1M AmSulfate, 5% Glycerol; Cryo 30% Glycerol, 20% PEG 5000, 0.1M BisTris pH 6.5, 0.1M AmSulfate, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |