1TQU

Golgi alpha-Mannosidase II In Complex With The Salacinol Analog Ghavamiol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	68.298, 108.957, 137.723
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.460 - 2.030
R-factor	0.132
Rwork	0.132
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hty
RMSD bond length	0.022
RMSD bond angle	2.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.047	0.176
Number of reflections	67857
<I/σ(I)>	40.18	9.7
Completeness [%]	99.8	97.1
Redundancy	7.5	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7.00