1TQI
Crystal Structure of A. Fulgidus Rio2 Serine Protein Kinase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97942, 0.97931, 0.99998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.588, 44.452, 62.603 |
| Unit cell angles | 90.00, 94.31, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.17029 |
| Rwork | 0.168 |
| R-free | 0.21173 |
| Structure solution method | MAD |
| Starting model (for MR) | Auto-sharp generated model |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.066 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 20608 | |
| <I/σ(I)> | 23.07 | 9.13 |
| Completeness [%] | 99.0 | 98.1 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.85 | 293 | PEG 900, sodium phosphate, sodium citrate, pH 3.85, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
