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1TQG

CheA phosphotransferase domain from Thermotoga maritima

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCHESS BEAMLINE F1
Synchrotron siteCHESS
BeamlineF1
Temperature [K]100
Detector technologyCCD
DetectorADSC QUANTUM 4
Wavelength(s)0.91612
Spacegroup nameP 2 2 21
Unit cell lengths27.381, 37.419, 87.542
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution15.000 - 0.980
R-factor0.1794
Rwork0.179
R-free0.21110
Structure solution methodMIR/MAD PHAING
RMSD bond length0.014
RMSD bond angle0.035
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASES
Refinement softwareSHELXL-97
Data quality characteristics
 Overall
Low resolution limit [Å]15.000
High resolution limit [Å]0.980
Number of reflections51414
Completeness [%]97.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP4.529828% PEG 8K, 0.1M NaOAc pH=4.5, and 0.2M NH4OAc, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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