1TQE
Mechanism of recruitment of class II histone deacetylases by myocyte enhancer factor-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-08-18 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 44.797, 66.930, 66.967 |
Unit cell angles | 76.67, 71.83, 71.81 |
Refinement procedure
Resolution | 30.000 - 2.700 |
Rwork | 0.263 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Structure of MEF2A and DNA complex pdb entry 1egw |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.040 | 0.227 |
Number of reflections | 17405 | |
<I/σ(I)> | 63 | 5.5 |
Completeness [%] | 91.3 | 95 |
Redundancy | 3.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.35 | 315 | BTP, PEG, NaCl, glycerol, MgCl2, CaCl2, pH 6.35, VAPOR DIFFUSION, HANGING DROP, temperature 315K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | BTP | ||
2 | 1 | 1 | PEG | ||
3 | 1 | 1 | H2O | ||
4 | 1 | 2 | NaCl | ||
5 | 1 | 2 | glycerol | ||
6 | 1 | 2 | MgCl2 | ||
7 | 1 | 2 | CaCl2 | ||
8 | 1 | 2 | H2O |