1TQB
Ovine recombinant PrP(114-234), VRQ variant in complex with the Fab of the VRQ14 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Detector technology | CCD |
| Collection date | 2003-08-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.047, 145.682, 43.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 2.550 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.610 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.050 | 0.164 |
| Number of reflections | 19725 | |
| <I/σ(I)> | 32.1 | 8.1 |
| Completeness [%] | 99.3 | 91.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | Sodium citrate 0.1M, PEG8000, Ammonium acetate 0.2M, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
