1TPT
THREE-DIMENSIONAL STRUCTURE OF THYMIDINE PHOSPHORYLASE FROM ESCHERICHIA COLI AT 2.8 ANGSTROMS RESOLUTION
Experimental procedure
Spacegroup name | P 43 21 2 |
Unit cell lengths | 133.000, 133.000, 67.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 2.800 |
R-factor | 0.28 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.800 * |
Total number of observations | 77819 * |
Number of reflections | 15034 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.5 * | took from Cook, W.J.,(1987) J. Biol. Chem., 262, 3788. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 52 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 35 (%) | |
3 | 1 | drop | citrate | 0.05 (M) | |
4 | 1 | reservoir | ammonium sulfate | 35 (%) | |
5 | 1 | reservoir | citrate | 0.05 (M) |