1TP5
Crystal structure of PDZ3 domain of PSD-95 protein complexed with a peptide ligand KKETWV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-23 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 89.978, 89.978, 89.978 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.650 - 1.540 |
R-factor | 0.1929 |
Rwork | 0.191 |
R-free | 0.22926 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1be9 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.558 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.630 |
High resolution limit [Å] | 1.540 | 1.540 |
Number of reflections | 18320 | |
Completeness [%] | 96.2 | 95.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 288 | 1.0 M sodium citrate, 0.1 M HEPES, 10 mM sodium chloride, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 288K |