1TOU
Crystal structure of human adipocyte fatty acid binding protein in complex with a non-covalent ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-16 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.829, 53.106, 31.383 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.000 |
R-factor | 0.20372 |
Rwork | 0.200 |
R-free | 0.27947 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.210 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.064 | 0.209 |
Number of reflections | 8483 | |
<I/σ(I)> | 17.9 | 4.4 |
Completeness [%] | 98.5 | 93.5 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG2000, DMSO, TRIS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |