1TO6
Glycerate kinase from Neisseria meningitidis (serogroup A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9790 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.767, 77.386, 101.436 |
Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
Resolution | 19.760 - 2.500 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.28300 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 34350 | |
Completeness [%] | 97.0 | 89.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 291 | 21% PEG MME 2K, 0.1M SODIUM CITRATE, 0.2M AMMONIUM SULFATE, pH 4.5, VAPOR DIFFUSION, temperature 291K |