1TO6
Glycerate kinase from Neisseria meningitidis (serogroup A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.767, 77.386, 101.436 |
| Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
| Resolution | 19.760 - 2.500 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.28300 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 34350 | |
| Completeness [%] | 97.0 | 89.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 291 | 21% PEG MME 2K, 0.1M SODIUM CITRATE, 0.2M AMMONIUM SULFATE, pH 4.5, VAPOR DIFFUSION, temperature 291K |
