1TO0
X-ray structure of Northeast Structural Genomics target protein sr145 from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.691, 70.553, 100.488 |
Unit cell angles | 90.00, 95.16, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vh0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | COMO |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.096 | 0.516 |
Number of reflections | 113561 | |
Completeness [%] | 96.0 | 77 |
Redundancy | 3.89 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 18-20 % PEG 3350, 0.2 M KCl, 50 mM TrisHCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |