1TN3
THE C-TYPE LECTIN CARBOHYDRATE RECOGNITION DOMAIN OF HUMAN TETRANECTIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 64.250, 64.250, 75.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.218 |
| Rwork | 0.215 |
| R-free | 0.26700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2msb ONE MONOMER |
| RMSD bond length | 0.016 |
| RMSD bond angle | 17.700 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 * | 0.221 * |
| Number of reflections | 10723 | |
| <I/σ(I)> | 11.4 | 3.4 |
| Completeness [%] | 97.2 | 98.3 |
| Redundancy | 4.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 * | Kastrup, J.S., (1997) Acta Crystallogr.,Sect.D, 53, 108. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.86 (mg/ml) | |
| 2 | 1 | drop | isopropanol | 2.86 (%) | |
| 3 | 1 | drop | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | drop | ethanol | 2.14 (%) | |
| 5 | 1 | reservoir | ethanol | 5 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 2.1 (M) |
