1TML

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF A THERMOPHILIC ENDOCELLULASE

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	43.350, 65.940, 43.410
Unit cell angles	90.00, 107.69, 90.00

Refinement procedure

Resolution	? - 1.800
R-factor	0.184
Rwork	0.184
RMSD bond length	0.014
RMSD bond angle	2.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.046 *
Total number of observations	79576 *
Number of reflections	21081 *
Completeness [%]	97.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-18 (mg/ml)
2	1	drop	MES	2 (mM)
3	1	drop		0.04 (%(w/v))
4	1	reservoir	disodium citrate	0.0056 (M)
5	1	reservoir		10 (mM)
6	1	reservoir	ammonium sulfate	12 (%)