1TMI
Structure of Thermotoga maritima S63A non-processing mutant S-adenosylmethionine decarboxylase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2003-08-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792,0.9793,0.9641 |
Spacegroup name | H 3 |
Unit cell lengths | 104.968, 104.968, 69.523 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.470 - 1.700 |
R-factor | 0.146 |
Rwork | 0.146 |
R-free | 0.18200 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.500 | 1.810 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 30984 | |
<I/σ(I)> | 30.7 | 4.7 |
Completeness [%] | 98.7 | 100 |
Redundancy | 5.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295.4 | ammonium formate, HEPES, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295.4K |