1TMC

THE THREE-DIMENSIONAL STRUCTURE OF A CLASS I MAJOR HISTOCOMPATIBILITY COMPLEX MOLECULE MISSING THE ALPHA3 DOMAIN OF THE HEAVY CHAIN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	48.230, 58.050, 109.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.207
Rwork	0.207
R-free	0.30400 *
RMSD bond length	0.008
RMSD bond angle	1.920
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.070 *	0.228 *
Completeness [%]	99.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	MES	25 (mM)
3	1	reservoir	PEG8000	10-20 (%)
4	1	reservoir	MES	25 (mM)