1TLZ
Tsx structure complexed with uridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 149.861, 149.861, 120.849 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.770 - 3.100 |
| R-factor | 0.299 |
| Rwork | 0.276 |
| R-free | 0.29900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 25887 | |
| Completeness [%] | 97.2 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 286 | PEG550 27-32%, sodium acetate 50 mM, glycerol 10%, uridine 100 mM, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
