1TLV
Structure of the native and inactive LicT PRD from B. subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 48.734, 48.734, 162.472 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.900 - 1.950 |
| R-factor | 0.269 |
| Rwork | 0.269 |
| R-free | 0.29200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 18537 | |
| <I/σ(I)> | 44.7 | 5.5 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 8.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.4 | 291 | 0.5-0.7 M potassium acetate, 0.1 M sodium citrate, pH 3.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
