1TLU
Crystal Structure of Thermotoga maritima S-adenosylmethionine decarboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2003-10-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9786 |
Spacegroup name | H 3 |
Unit cell lengths | 104.994, 104.994, 69.695 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.470 - 1.550 |
R-factor | 0.155 |
Rwork | 0.152 |
R-free | 0.18700 |
Structure solution method | SINGLE WAVELENGTH |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.470 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 39077 | |
<I/σ(I)> | 29.3 | 3.1 |
Completeness [%] | 98.2 | 98.2 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295.4 | ammonium formate, HEPES, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295.4K |