1TLT
Crystal Structure of a Putative Oxidoreductase (VIRULENCE FACTOR mviM HOMOLOG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 96.856, 96.856, 380.817 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.910 - 2.700 |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.29400 |
Structure solution method | SAD |
Starting model (for MR) | Experimental electron density. |
RMSD bond length | 0.011 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 29879 | |
<I/σ(I)> | 25.75 | 3.47 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 13.31 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 1.4M Ammonium Sulfate, 0.1M Na Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 1.4M Ammonium Sulfate, 0.1M Na Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 1.4M Ammonium Sulfate, 0.1M Na Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |