1TLK
X-RAY STRUCTURE DETERMINATION OF TELOKIN, THE C-TERMINAL DOMAIN OF MYOSIN LIGHT CHAIN KINASE, AT 2.8 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.400, 64.400, 59.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.184 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 3.000 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 * |
| High resolution limit [Å] | 2.700 * |
| Rmerge | 0.050 * |
| Total number of observations | 15144 * |
| Number of reflections | 4061 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 8 (%) | |
| 2 | 1 | 1 | sodium azide | 0.5 (mM) | |
| 3 | 1 | 1 | succinic acid | 50 (mM) | |
| 4 | 1 | 1 | potassium hydroxide | 7-12 (mg(telokin/ml)) | |
| 5 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 6 | 1 | 1 | potassium phosphate | 10 (mM) |
