1TLJ
Crystal Structure of Conserved Protein of Unknown Function SSO0622 from Sulfolobus solfataricus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | SBC-3 |
Wavelength(s) | 0.97927,0.97948, 0.96404 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 92.114, 92.114, 110.907 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.2489 |
Rwork | 0.223 |
R-free | 0.27160 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.082 | 0.399 |
Number of reflections | 25954 | |
<I/σ(I)> | 8.3 | 2.99 |
Completeness [%] | 99.4 | 100 |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 5000MME, ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |