1TL2

TACHYLECTIN-2 FROM TACHYPLEUS TRIDENTATUS (JAPANESE HORSESHOE CRAB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	289
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	89.460, 89.460, 73.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.000
Rwork	0.162
R-free	0.20200
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.600 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.200	2.430
High resolution limit [Å]	2.000	2.030
Rmerge	0.074	0.171
Total number of observations	83799 *
Number of reflections	20896
Completeness [%]	93.7	92.2
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.1 *	20 *	pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Mops	10 (mM)
3	1	drop	GlcNAc	5 (mM)
4	1	reservoir	sodium acetate	100 (mM)
5	1	reservoir	sodium formate	2.0 (M)