1TKY
Crystal structure of the editing domain of threonyl-tRNA synthetase complexed with seryl-3'-aminoadenosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-24 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9330 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.563, 45.429, 116.532 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.520 - 1.480 |
R-factor | 0.203 |
Rwork | 0.201 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qf6 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.520 | 1.520 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.071 | 0.228 |
Number of reflections | 39904 | |
Completeness [%] | 98.8 | 93.4 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 281 | PEG 4000, Tris.cl, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |