1TKU
Crystal Structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans in complex with Ribulose-5-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-07-21 |
Detector | MARRESEARCH |
Wavelength(s) | 1.542 |
Spacegroup name | P 1 |
Unit cell lengths | 40.200, 47.900, 59.800 |
Unit cell angles | 66.40, 72.30, 89.90 |
Refinement procedure
Resolution | 20.000 - 1.660 |
Rwork | 0.184 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g57 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.672 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.032 | 0.174 |
Number of reflections | 39461 | |
<I/σ(I)> | 22.2 | |
Completeness [%] | 77.3 | 28.7 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | Mes/NaOH, PEG8000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |