1TKF

Streptomyces griseus aminopeptidase complexed with D-tryptophan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-05
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.93930
Spacegroup name	P 41 21 2
Unit cell lengths	60.606, 60.606, 144.186
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.200
Rwork	0.130
R-free	0.16810
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.014
RMSD bond angle	0.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	83789
<I/σ(I)>	13.5
Completeness [%]	99.0	91.5
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	PEG 4000, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K