1TKE

Crystal structure of the editing domain of threonyl-tRNA synthetase complexed with serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	P 21 21 21
Unit cell lengths	43.697, 44.913, 116.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.450 - 1.460
R-factor	0.18
Rwork	0.178
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qf6
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.500	1.520
High resolution limit [Å]	1.460	1.460
Rmerge	0.041	0.129
Number of reflections	40336
Completeness [%]	99.6	98.7
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	281	PEG 4000, Tris.cl, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K