1TKC

SPECIFICITY OF COENZYME BINDING IN THIAMIN DIPHOSPHATE DEPENDENT ENZYMES: CRYSTAL STRUCTURES OF YEAST TRANSKETOLASE IN COMPLEX WITH ANALOGS OF THIAMIN DIPHOSPHATE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	76.300, 113.300, 160.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.152
Rwork	0.152
RMSD bond length	0.015
RMSD bond angle	3.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.053 *
Total number of observations	56543 *
Number of reflections	24737 *
Completeness [%]	72.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	PEG6000	7.5 (%)
3	1	drop	glycylglycine	25 (mM)
4	1	drop	thiamine-PP	2 (mM)
5	1	reservoir	glycylglycine	50 (mM)
6	1	reservoir	thiamine-PP	2 (mM)