1TK6
Iron-oxo clusters biomineralizing on protein surfaces. Structural analysis of H.salinarum DpsA in its low and high iron states
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.731 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 91.110, 91.110, 150.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 158.110 - 2.200 |
| R-factor | 0.17651 |
| Rwork | 0.172 |
| R-free | 0.23543 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tjo |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.254 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 158.110 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.110 |
| Number of reflections | 36571 |
| <I/σ(I)> | 11.11 |
| Completeness [%] | 98.7 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG400, 1M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
