1TJW
Crystal Structure of T161D Duck Delta 2 Crystallin Mutant with bound argininosuccinate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.996, 98.806, 106.387 |
Unit cell angles | 90.00, 101.34, 90.00 |
Refinement procedure
Resolution | 41.760 - 2.000 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.22500 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1hy1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.800 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 128293 | |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 14% PEG2000 MME, 350 mM magnesium chloride, 100 mM HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |