1TJU

Crystal Structure of T161S Duck Delta 2 Crystallin Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	93.318, 98.896, 106.996
Unit cell angles	90.00, 101.53, 90.00

Refinement procedure

Resolution	44.700 - 2.100
R-factor	0.178
Rwork	0.178
R-free	0.22100
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1hy1
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	106162
Completeness [%]	93.2	83.8
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	14% PEG2000 MME, 300 mM magnesium chloride, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K