1TJT
X-ray structure of the human alpha-actinin isoform 3 at 2.2A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.310, 38.540, 65.480 |
| Unit cell angles | 90.00, 99.72, 90.00 |
Refinement procedure
| Resolution | 34.610 - 2.190 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QAG for CH1 domain and 1BKR for CH2 domain |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.610 | 2.330 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.089 | 0.186 |
| Number of reflections | 11674 | |
| Completeness [%] | 97.4 | 93.1 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | peg 4000, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
