1TJO
Iron-oxo clusters biomineralizing on protein surfaces. Structural analysis of H.salinarum DpsA in its low and high iron states
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 91.110, 91.110, 150.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.600 |
R-factor | 0.16808 |
Rwork | 0.165 |
R-free | 0.21039 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Dps-protein from E. coli - PDB entry 1DPS |
RMSD bond length | 0.017 |
RMSD bond angle | 1.509 |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 158.000 | 1.641 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.074 | |
Number of reflections | 92251 | |
<I/σ(I)> | 10 | |
Completeness [%] | 96.5 | 99.1 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG400, 1M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |