1TJN

Crystal structure of hypothetical protein af0721 from Archaeoglobus fulgidus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-22
Detector	SBC-3
Wavelength(s)	0.979374,0.979238,0.964108
Spacegroup name	I 41 2 2
Unit cell lengths	102.460, 102.460, 59.843
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.460 - 2.010
R-factor	0.21952
Rwork	0.217
R-free	0.26006
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.299
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.040
High resolution limit [Å]	2.000	2.010
Rmerge	0.060
Number of reflections	10173
Completeness [%]	85.2	82.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	297	1.9 M AMMONIUM SULFATE, 0.1 M HEPES, 4% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 297K