1TJK
Crystal structure of the complex formed between group II phospholipase A2 with a designed pentapeptide, Phe- Leu- Ser- Thr- Lys at 1.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-04-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8030 |
| Spacegroup name | P 43 |
| Unit cell lengths | 52.293, 52.293, 47.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.990 - 1.250 |
| R-factor | 0.18454 |
| Rwork | 0.184 |
| R-free | 0.20273 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1skg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.725 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 35680 | |
| Completeness [%] | 100.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | Ammonium Sulphate, Polyethylene glycol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
