1TJF
The crystal structure of the N-terminal domain of CAP indicates variable oligomerisation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-07 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.240, 75.060, 162.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.780 - 2.210 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s0p |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.220 |
| Rmerge | 0.072 | 0.303 |
| Number of reflections | 21214 | |
| <I/σ(I)> | 8.4 | 2.5 |
| Completeness [%] | 98.8 | 94.3 |
| Redundancy | 6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 283 | ammonium sulphate, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
