1TJ7
Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.134 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 121.469, 121.469, 255.251 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.440 |
| R-factor | 0.16942 |
| Rwork | 0.167 |
| R-free | 0.21742 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k7w |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.537 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.530 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.099 | 0.401 |
| Number of reflections | 42164 | |
| <I/σ(I)> | 20.7 | 6.06 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 14.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | Ammonium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
