1TJ5

X-Ray structure of the Sucrose-Phosphatase (SPP) from Synechocystis sp. PCC6803 in complex with sucrose and phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-19
Detector	ADSC QUANTUM 210
Wavelength(s)	0.976
Spacegroup name	P 65 2 2
Unit cell lengths	68.750, 68.750, 268.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.189
R-free	0.22000
Structure solution method	rigid body
Starting model (for MR)	1tj3
RMSD bond length	0.005
RMSD bond angle	1.390
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.195
Number of reflections	19930
<I/σ(I)>	10.69
Completeness [%]	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	sodium Formate, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K