1TIP
THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-05 |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.230, 56.520, 90.900 |
| Unit cell angles | 90.00, 94.48, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.28600 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 23.466 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 * |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.033 * |
| Number of reflections | 20142 |
| Completeness [%] | 78.3 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | Lee, Y.H., (1996) Biochemistry, 35, 6010. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 10 | 1 | drop | water | ||
| 2 | 1 | drop | PEG4000 | 15 (%) | |
| 3 | 1 | drop | sodium phosphate | 5 (mM) | |
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | drop | glycerol | 2.5 (%) | |
| 6 | 1 | reservoir | PEG4000 | 30 (%) | |
| 7 | 1 | reservoir | sodium phosphate | 10 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 9 | 1 | reservoir | glycerol | 5 (%) |
