1TIO
HIGH PACKING DENSITY FORM OF BOVINE BETA-TRYPSIN IN CYCLOHEXANE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Collection date | 1996-04-15 |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.540, 58.260, 67.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.930 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bty |
RMSD bond length | 0.007 |
RMSD bond angle | 26.000 * |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 64.000 |
High resolution limit [Å] | 1.930 |
Rmerge | 0.088 |
Total number of observations | 34500 * |
Number of reflections | 14880 |
<I/σ(I)> | 12 |
Completeness [%] | 88.0 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 * | 20 * | HANGING DROP METHOD: THE PROTEIN WAS DISSOLVED IN 10 MM HAC-NAAC BUFFER (PH 4.1) PRECIPITANT AND RESERVOIR SOLUTION WERE 0.5 M NA2SO4 AND 0.1 M SODIUM CACODYLATE-HCL BUFFER CONTAINING 15% (W/V) MGSO4 AND 1.5% (V/V) MPD AT PH 6.2., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | HAc-NaAc | 10 (mM) | |
3 | 1 | drop | benzamidine | 16 (mM) | |
4 | 1 | drop | 1 (mM) | ||
5 | 1 | reservoir | sodium cacodylate-HCl | 0.1 (M) | |
6 | 1 | reservoir | 15 (%(w/v)) | ||
7 | 1 | reservoir | 0.5 (M) | ||
8 | 1 | reservoir | MPD | 1.5 (%(v/v)) |