1TIC

CONFORMATIONAL LABILITY OF LIPASES OBSERVED IN THE ABSENCE OF AN OIL-WATER INTERFACE: CRYSTALLOGRAPHIC STUDIES OF ENZYMES FROM THE FUNGI HUMICOLA LANUGINOSA AND RHIZOPUS DELEMAR

Experimental procedure

Spacegroup name	C 1 21 1
Unit cell lengths	92.770, 128.860, 78.350
Unit cell angles	90.00, 135.82, 90.00

Refinement procedure

Resolution	7.500 - 2.600
R-factor	0.16
RMSD bond length	0.016
RMSD bond angle	0.056
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.600 *
Rmerge	0.088 *
Completeness [%]	87.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.2 (%)
2	1	reservoir	ammonium sulfate	40 (%)
3	1	reservoir	sodium acetate	100 (mM)
4	1	reservoir	MPD		0.020ml
5	1	reservoir	N,N-dimethyl-octadecylamine-N-oxide	0.05 (mM)