1THX

THIOREDOXIN-2

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-11-22
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	40.480, 39.780, 60.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 1.600
R-factor	0.174 *
Rwork	0.175
R-free	0.22900
RMSD bond length	0.009 *
RMSD bond angle	1.630 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000 *
High resolution limit [Å]	1.600 *	1.600 *
Rmerge	0.035
Total number of observations	34399 *
Number of reflections	10467
Completeness [%]	78.6	49.5 *
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG1450	20-25 (%(w/w))	can be replaced with PEG-mono methyl ether 2000
3	1	reservoir	MES	100 (mM)