1TGR
Crystal Structure of mini-IGF-1(2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-30 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.860, 61.980, 70.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.420 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.22400 |
Structure solution method | SAD or SIRAS |
RMSD bond length | 0.016 |
RMSD bond angle | 1.621 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.450 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.096 | 0.376 |
Number of reflections | 23375 | |
<I/σ(I)> | 16.7 | 4.6 |
Completeness [%] | 99.6 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.65 | 289 | Citrate, ethanol, pH 6.65, VAPOR DIFFUSION, HANGING DROP, temperature 289K |