1TGO
THERMOSTABLE B TYPE DNA POLYMERASE FROM THERMOCOCCUS GORGONARIUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.110, 105.220, 154.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.500 |
R-factor | 0.209 * |
Rwork | 0.209 |
R-free | 0.27100 |
Structure solution method | MIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.417 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | |
High resolution limit [Å] | 2.500 | |
Rmerge | 0.071 * | 0.262 * |
Total number of observations | 482448 * | |
Number of reflections | 30451 | |
Completeness [%] | 0.9 | 86 * |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8.2 * | 4 * | pH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 20 (mM) | |
3 | 1 | drop | 2-mercaptoethanol | 10 (mM) | |
4 | 1 | drop | 500 (mM) | ||
5 | 1 | reservoir | Tris | 100 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 2.0 (M) |