1TGK
HUMAN TRANSFORMING GROWTH FACTOR BETA 3, CRYSTALLIZED FROM PEG 4000
Experimental procedure
Detector technology | DIFFRACTOMETER |
Collection date | 1992-12-23 |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 77.800, 77.800, 143.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 3.300 |
R-factor | 0.228 |
Rwork | 0.228 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.920 |
Data reduction software | ROTAVATA-AGROVATA |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9999.000 * | |
High resolution limit [Å] | 3.300 * | 3.300 * |
Rmerge | 0.132 | |
Number of reflections | 4232 | |
Completeness [%] | 100.0 | 100 * |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.4 * | 20-22 * | PEG 4000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1.5-3 (mg/ml) | |
2 | 1 | drop | acetic acid | 5 (mM) | |
3 | 1 | drop | PEG4000 | 15 (%(w/v)) | |
4 | 1 | drop | magnesium chloride | 100 (mM) | |
5 | 1 | drop | Tris-HCl | 50 (mM) | pH8.4 |
6 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
7 | 1 | reservoir | magnesium chloride | 200 (mM) | |
8 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.4 |